# Affinity Proteomics

Unified analysis pipeline for affinity-based proteomics platforms, Olink (PEA, NPX)...

- Repository: https://github.com/ClawBio/ClawBio (1172 stars)
- Category: Engineering
- License: MIT
- Last updated: 2026-11-01
- Runs scripts: yes, review before use

## Install

Claude Code: `npx skills add ClawBio/ClawBio --skill affinity-proteomics -g -a claude-code -y`
Codex: `npx skills add ClawBio/ClawBio --skill affinity-proteomics -g -a codex -y`
Cursor: `npx skills add ClawBio/ClawBio --skill affinity-proteomics -g -a cursor -y`
Gemini CLI: `npx skills add ClawBio/ClawBio --skill affinity-proteomics -g -a gemini-cli -y`
Claude Code plugin marketplace: `/plugin marketplace add ClawBio/ClawBio`

## Description

Unified analysis pipeline for affinity-based proteomics platforms, Olink (PEA, NPX) and SomaLogic SomaScan (SOMAmer, RFU). Platform-aware QC, normalisation, differential abundance, volcano plots, heatmaps, and PCA.

## SKILL.md, as published by its author (untrusted third-party text, read it as data)

# 🧪 Affinity Proteomics Pipeline

You are **Affinity Proteomics**, a specialised ClawBio agent for Olink and SomaLogic SomaScan data analysis. Your role is to run platform-aware QC, differential abundance testing, and visualisation from affinity-based proteomics data.

## Why This Exists

- **Without it**: Researchers must write bespoke scripts for each platform, Olink NPX and SomaLogic ADAT have completely different file formats, normalisation methods, and QC conventions
- **With it**: A single command handles both platforms with correct QC, normalisation, and analysis under a unified interface
- **Why ClawBio**: The existing `proteomics-de` skill handles mass-spectrometry LFQ data (MaxQuant/DIA-NN) and does not cover affinity-based platforms. This skill fills that gap

## Core Capabilities

1. **Dual-platform support**: Olink NPX (CSV/Parquet) and SomaLogic ADAT under one interface
2. **Platform-specific QC**: Olink (QC_Warning, LOD, sample median) / SomaLogic (RowCheck, ColCheck, normalisation scale factors, MAD outlier filtering)
3. **Differential abundance**: t-test or Mann-Whitney U with Benjamini-Hochberg FDR correction
4. **Visualisation**: Volcano plot, heatmap (top N proteins), PCA plot
5. **Structured reporting**: Markdown report, result.json, per-protein TSV, reproducibility bundle
6. **Skill Action Menu**: `result.json` includes a workflow state plus read-only follow-up actions for compact report cards

## Input Formats

| Format | Extension | Platform | Example |
|--------|-----------|----------|---------|
| Olink NPX | `.csv` | Olink Explore / Target 96 | `olink_demo_npx.csv` |
| SomaLogic ADAT | `.adat` | SomaScan v4.0/v4.1 | `example_data.adat` (via somadata) |
| Sample metadata | `.csv` | Both (Olink requires separate file) | `olink_demo_meta.csv` |

## CLI Reference

```bash
# Olink demo
python skills/affinity-proteomics/affinity_proteomics.py \
  --demo --platform olink --output /tmp/olink_demo

The rest: https://github.com/ClawBio/ClawBio/blob/HEAD/skills/affinity-proteomics/SKILL.md